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Postdoctoral Researcher in Chromatin and Gene Expression

Postdoctoral Researcher in Chromatin and Gene Expression

The Institute

The Centre for Genomic Regulation (CRG) is an international biomedical research institute of excellence, based in Barcelona, Spain, with more than 400 scientists from 44 countries. The CRG shares principles of an interdisciplinary, motivated and creative scientific team that is supported by high-end and innovative technologies and a flexible and efficient administration.

In November 2013, the Centre for Genomic Regulation (CRG) received the 'HR Excellence in Research' logo from the European Commission. This is a recognition of the Institute's commitment to developing an HR Strategy for Researchers, designed to bring the practices and procedures in line with the principles of the European Charter for Researchers and the Code of Conduct for the Recruitment of Researchers (Charter and Code).

Please, check out our Recruitment Policy

The role


Working as part of an ongoing ERC-Proof of Concept grant, the successful candidate will carry out proposed experiments in line with the aims of the funding. Successful candidate will be required to carry out basic cell culture experiments in both 2D and 3D cancer cell culture; assessing both basic phenotypic, behavioural and gene expression changes (at local and global level) following the treatment of cells with putative combinations of cancer inhibitors.


About the team/ lab/ department

Using steroid hormone action in breast cancer cells as a model, the group explores how eukaryotic cells respond to external cues; specifically, how signals are transduced to the nucleus and modulate chromatin structure and gene expression. Previously, we found that gene regulation by progestins involves the communication of membrane-attached and nuclear progesterone receptor (PR) associated with ERK and MSK1 kinases and involves two consecutive cycles of chromatin remodelling: a very rapid displacement of histone H1 mediated by NURF, ASCOM/MLL2, CDK2, PARP1 and KDM5, followed by a slower displacement of histones H2A/H2B mediated by PCAF and BAF. The Mb topological chromatin domains (TADs) conserved in the human genome behave as units of hormone response that undergo structural changes in response to hormone. Unexpectedly we found that the ATP required for the associated chromatin remodelling is generated in the nucleus from ADP-Ribose and PPi by the enzyme NUDIX5. Our final aim is to integrate the signalling network with the changes in the topological organization of chromatin and the transcriptional response to generate a multidimensional network that will reveal the logic of the hormonal control of cell proliferation. Ultimately resulting in new therapeutic options for breast cancer patients.

Whom would we like to hire?

Professional experience

Must Have

  • You have over 3 years postdoctoral experience in gene expression and chromatin structure analysis.
  • You have proven experience in mammalian cell culture both in 2D and 3D dimensions, experience in in-vitro enzyme activity assays. Chromatin immunoprecipitation, expression analysis by qRT-PCR, RNA-seq.
  • Proven track record of working independently and part of a team.

Desirable but not required/ Nice to have

  • Bioinformatics experience

Education and training

  • You hold a Phd in Biochemistry/ Molecular Biology or similar.

Languages

  • You are proficient in English

Technical skills

  • You have advanced MS Office skills.

Competences

  • Highly developed organization skills
  • Presentation skills
  • Detail orientated

The Offer

  • Contract duration: 6 months
  • Estimated annual gross salary: Salary is commensurate with qualifications and consistent with our pay scales.
  • Target start date: September 2019

We provide a highly stimulating environment with state-of-the-art infrastructures, and unique professional career development opportunities.

We offer and promote a diverse and inclusive environment and welcomes applicants regardless of age, disability, gender, nationality, race, religion or sexual orientation.

The CRG is committed to reconcile a work and family life of its employees and are offering extended vacation period and the possibility to benefit from flexible working hours.

Application Procedure
All applications must include:

  1. A motivation letter addressed to Dr Miguel Beato.
  2. A complete CV including contact details.
  3. Contact details of two referees.

All applications must be addressed to Dr. Miguel Beato and be submitted online on through the "Apply" button below

Deadline: Please submit your application by 26th of August, 2019