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Laboratory technician in the Hematopoietic stem cells, transdifferentiation and reprogramming Lab

Laboratory technician in the Hematopoietic stem cells, transdifferentiation and reprogramming Lab

The Institute

The Centre for Genomic Regulation (CRG) is an international biomedical research institute of excellence, based in Barcelona, Spain, with more than 400 scientists from 44 countries. The CRG shares principles of an interdisciplinary, motivated and creative scientific team that is supported by high-end and innovative technologies and a flexible and efficient administration.

In November 2013, the Centre for Genomic Regulation (CRG) received the 'HR Excellence in Research' logo from the European Commission. This is a recognition of the Institute's commitment to developing an HR Strategy for Researchers, designed to bring the practices and procedures in line with the principles of the European Charter for Researchers and the Code of Conduct for the Recruitment of Researchers (Charter and Code).

The role 

The lab technician will help performing a number of molecular biological techniques, including RNAseq, ATACseq and Hi-C. She/he will also genotype mice and perform tissue culture experiments. Finally, she/he will assist researchers at various tasks.

About the lab

Our laboratory has developed a 100% efficient transdifferentiation system, namely the conversion of B cells into functional macrophages by the overexpression of the transcription factor C/EBPa. Using this system, we are studying how C/EBPa activates the macrophage gene expression program and concomitantly silences the B cell program. We are also interested in how C/EBPa collaborates with pluripotency factors in the generation of induced pluripotent cells.

This work will enhance our understanding of normal differentiation and its aberrations in blood cell cancers. A better understanding of cell conversion processes might also help designing new protocols for the improved reprogramming of somatic cells that could become useful for tissue replacement.

Whom would we like to hire?

Professional experience 

Must Have

  • You have 2 years of experience in/with tissue culture, mouse genotyping, basic molecular biology techniques
  • You have proven experience in/ with mouse genotyping, qPCR, cell culture, Western blots, Immunofluoresence

Desirable but not required/ Nice to have

  • You have expertise in RNA fish in single cell ESCs
  • You have super-resolution (STORM) imaging sample preparation

Education and training

  • You hold a degree in Technician of analysis and quality control

Languages

  • You are proficient in Catalan, Spanish and English

Technical skills

  • You have advanced MS Office skills
  • You have cellular and microbiological skills
  • You have knowledge of DNA & RNA techniques
  • You have imaging Skills (confocal microscope)
  • You have protein Analysis Techniques
  • You have expertise with platforms such Oracle or Labguru

Competences 

  • You have highly developed organization skills
  • You perform carefully executed experiments
  • You are flexible in performing different tasks
  • You are willing to work outside of the official schedule if the work so requires

The Offer – Working Conditions

  • Contract duration: 2 years
  • Estimated annual gross salary: Salary is commensurate with qualifications and consistent with our pay scales.
  • Target start date: 15 July, 2020

We provide a highly stimulating environment with state-of-the-art infrastructures, and unique professional career development opportunities. To check out our training and development portfolio, please visit our website in the training section.

We offer and promote a diverse and inclusive environment and welcomes applicants regardless of age, disability, gender, nationality, race, religion or sexual orientation.

The CRG is committed to reconcile a work and family life of its employees and are offering extended vacation period and the possibility to benefit from flexible working hours.

Application Procedure

All applications must include:

  1. A motivation letter addressed to Dr. Thomas Graf
  2. A complete CV including contact details.
  3. Contact details of two referees.

All applications must be addressed to Dr. Thomas Graf and be submitted online through the "Apply" button below.

Selection Process 

  • Pre-selection: The pre-selection process will be based on qualifications and expertise reflected on the candidates CVS. It will be merit-based.
  • Interview: Preselected candidates will be interviewed by the Hiring Manager of the position and a selection panel if required.
  • Offer Letter: Once the successful candidate is identified the Human Resources department will send a Job Offer, specifying the start day, salary, working conditions, among other important details.

Deadline: Please submit your application by 10th June, 2020.

El contrato puede estar financiado por el Ministerio de Ciencia e Innovación y la Agencia Estatal de Investigación y cofinanciado con fondos FEDER, referencia oficial del proyecto: PID2019-109354GB-I00.

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